| 產(chǎn)品名稱 |
SiHa |
| 商品貨號(hào) |
B161694 |
| Organism |
Homo sapiens, human |
| Tissue |
cervix |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
2 Cells contain human papilloma virus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
grade II,squamous cell carcinoma |
| Age |
55 years adult |
| Gender |
female |
| Ethnicity |
Asian |
| Applications |
This cell line is a suitable transfection host. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal number = 69; range = 51 to 72.
This is a hypertriploid human cell line with the modal chromosome number of 71, occurring in 24% of cells. Most cells had the chromosome numbers distributed between 69 and 72. Polyploid cells occurred at 7.6%. Fifteen or more marker chromosomes were common to most cells. Among them are dup(2) (q22q31) and del(2) (q31) which probably resulted from the balanced translocation between two N2s. Most cells had two copies of del(2). M2 is an A3-sized acrocentric. M13 is a minute submetacentric with 1-3 copies per cell. Origins of both M2 and M13 are not identified. There were two copies of normal X chromosomes. N2 was absent and probably was replaced by dup(2) and del(2). |
| Derivation |
This line was established from fragments of a primary tissue sample obtained after surgery from a Japanese patient. |
| Clinical Data |
55 years adult Asian female This line was established from fragments of a primary tissue sample obtained after surgery from a Japanese patient. |
| Oncogene |
p53 +; pRB + |
| Genes Expressed |
Oncogenes: p53 +; pRB + |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice; forms poorly differentiated epidermoid carcinoma (grade III) |
| Comments |
Electron microscopic observations revealed presence of typical desmosomes at the cell junctions and an abundance of tonofilaments in the cytoplasm. Mycoplasma contamination was detected and eliminated in 1975. The line is reported to contain an integrated human papillomavirus type 16 genome (HPV-16, 1 to 2 copies per cell). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing |
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
- Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
-
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 12 D13S317: 11 D16S539: 12 D5S818: 9 D7S820: 10 THO1: 6,9 TPOX: 8 vWA: 14,17 |
| Isoenzymes |
AK-1, 1 ES-D, 2 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1 |
| Name of Depositor |
Y Ito |
| Deposited As |
Homo sapiens |
| References |
Annunziata C, et al. Distinct profiles of TERT promoter mutations and telomerase expression in head and neck cancer and cervical carcinoma. Int J Cancer 143(5): 1153-1161, 2018. PubMed: 29603728
Baker CC, et al. Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. J. Virol. 61: 962-971, 1987. PubMed: 3029430
Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153
Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217
Friedl F, et al. Studies on a new human cell line (SiHa) derived from carcinoma of uterus. I. Its establishment and morphology. Proc. Soc. Exp. Biol. Med. 135: 543-545, 1970. PubMed: 5529598
Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218
Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105
Olive PL, Banath JP. Multicell spheroid response to drugs predicted with the comet assay. Cancer Res. 57: 5528-5533, 1997. PubMed: 9407963
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| Cross References |
Nucleotide (GenBank) :
AF001600
Human papillomavirus type 16 integrated SiHa HPV-16 variant DNA, capsid protein gene, complete cds, and flanking cellular DNA.
Nucleotide (GenBank) :
AF001599
Human papillomavirus type 16 integrated SiHa HPV-16 variant, replication protein gene, complete cds, and flanking cellular sequence.
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