Restriction digests of the clone give the following sizes (kb): EcoRI--4.1, 1.25, 0.41; HindIII--3.9, 1.9; PstI--4.4, 1.2. The insert contains the following restriction sites (approximate kb from the 5' end): SstI--0.01; PvuII--0.14, 0.72; EcoRI--0.44, 0.86; HindIII--1.09. Contains the complete coding sequence (nt 91-633). Used to map the human gene by in situ hybridization. |