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Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 5.6180000305175780

DESCRIPTION OF VECTOR:
Intact vector size: 5.618
Type of vector: plasmid
Cloning sites: EcoRI ClaI
Polylinker sites: HindIII NcoI EcoRI ClaI HindIII
Construction: pBact16, pUC18, c-myc
Host range: Escherichia coli; mammalian cells
Features (with orientation and position when available):
promoter: beta-actin, ->
restriction site: HindIII
other: Kozak consensus sequence
restriction site: NcoI
other: c-myc epitope sequence, ->
restriction site: EcoRI
restriction site: ClaI
restriction site: HindIII
terminator: SV40 polyadenylation
replicon: pMB1
marker(s): ampR, <-

Vector: pBact-myc (plasmid)

Promoters: Promoter beta-actin

Construction: pBact16, pUC18, c-myc

Marker(s):ampR

Construct size (kb): 5.618000030517578

Features: marker(s): ampR
other: Kozak consensus sequence
other: c-myc epitope sequence
promoter: beta-actin
replicon: pMB1
restriction site: ClaI
restriction site: EcoRI
restriction site: HindIII
restriction site: NcoI
terminator: SV40 polyadenylation

expression vector

shuttle vector

vector permitting construction of fusion proteins

Restriction digests of the clone give the following sizes (kb): HindIII--5.6, PstI--4.8, 0.45, 0.35; EcoRI--5.6.

Shuttle expression vector allowing production of a fusion protein containing an amino terminal c-myc epitope tag (FQKLISEEDLN), that can be used for immunological detection of the recombinant protein in transfected cells.

Temperature: 37.0°C