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Distribution host: Escherichia coli DH5alpha

Size (kb): 6.4000000953674320

DESCRIPTION OF VECTOR:
Intact vector size: 6.400
Type of vector: plasmid
Cloning sites: NotI SacI EcoRI KpnI BamHI XbaI SalI PstI SphI HindIII NotI
Polylinker sites: NotI SacI EcoRI KpnI BamHI XbaI SalI PstI SphI HindIII NotI
Other unique sites: NcoI SfiI SpeI
Construction: pCR-XL-TOPO
Features (with orientation and position when available):
primer site: M13 (forward, reverse)
promoter: lacZalpha
replicon: ColE1, ORFs
marker(s): kanamycin, zeocin

Vector: pBSV2 (plasmid)

Promoters: Promoter lacZalpha

Construction: pCR-XL-TOPO

Marker(s):kanR

Construct size (kb): 6.400000095367432

Features: marker(s): kanamycin, zeocin
primer site: M13 (forward, reverse)
promoter: lacZalpha
replicon: ColE1, ORFs

cloning vector

Restriction digests of the clone give the following sizes (kb): BamHI--6.4; EcoRI--4.2, 2.2; HindIII--6.4.

The plasmid pBSV2 is a shuttle vector capable of moving between E. coli and the Lyme disease spirochete, Borrelia burgdorferi.

Temperature: 37.0°C