Comments |
Restriction digests of the clone give the following sizes (kb): PstI--3.2, 0.86, 0.33; HindIII--2.5, 1.7, 0.69; BamHI--4.4; EcoRI--2.5, 1.95; BglII--4.4. Clones 2BP5 (ATCC 61332), HMJI (ATCC 61330), and Fr3-42 are three independent, single-copy probes from cosmid 2B. They are arranged on the chromosome in the following order: centromere, 2BP5, HMJI, Fr3-42, telomere. |