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Distribution host: Escherichia coli DH10B

Size (kb): 4.9000000953674320

DESCRIPTION OF VECTOR:
Intact vector size: 4.900
Type of vector: plasmid
Cloning sites: SmaI
Polylinker sites:
Construction: P15A ori, sacB, RP4, gtmR
Host range: broad host range; Escherichia coli
Features (with orientation and position when available):
replicon: oriV, p15A
other: traJ, <-
marker(s): sacB, ->
replicon: oriT
marker(s): gtmR, <-

Vector: pJQ200 (plasmid)

Construction: P15A ori, sacB, RP4, gtmR

Marker(s):sacB,gtmR

Construct size (kb): 4.900000095367432

Features: marker(s): gtmR
marker(s): sacB
other: traJ
replicon: oriT
replicon: oriV, p15A

integrating vector

vector permitting construction of fusion proteins

vector permitting positive selection for integration

vector permitting visual detection of recombinants

Restriction digests of the clone give the following sizes (kb): SmaI--5.2; EcoRI--5.2; HindIII--5.2.

Replicates only in enterobacteria, and so functions as a suicide vector in other Gram negative hosts.

A single BglII site within the gentamicin resistance gene (gtmR) allows insertion of other resistance genes (e.g. as a BamHI cassette).

When induced by media containing 5% sucrose, sacB is lethal in a wide range of Gram negative bacteria, and thus permits selection for loss of the vector.

Suicide vector permitting gene replacement and mobilization into a wide range of Gram negative bacteria. Allows positive selection for integration.

The order of the major features of the plasmid is: gtmR - SmaI - P15A - traJ - oriT - sacB.

Temperature: 37.0°C