国产精品乱视频,国产精品va久久久久久久,2021国产精品视频网站,91久久成人免费,乱色熟女少妇一区二区,天天干夜夜操夜夜操,麻豆久久樱花一区二区av,国产高清久久久久

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > CHLA-04-ATRT
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
CHLA-04-ATRT
CHLA-04-ATRT
規(guī)格:
貨期:
編號:B224247
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 CHLA-04-ATRT
商品貨號 B224247
Organism Homo sapiens, human
Tissue Brain (lateral and third ventricle)
Product Format frozen
Morphology rounded
Culture Properties single cells and clusters in suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Atypical Teratoid Rhabdoid Tumor (ATRT)
Age 20 months
Gender Male
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a human diploid cell line of male origin in which most of the examined cells contained the karyotypically normal male chromosome complement, 46,XY.
Images CRL-3036 Micrograph
Derivation CHLA-04-ATRT was established from a 20 month old boy with recurrent atypical teratoid rhabdoid tumor (ATRT) with wide dissemination in the brain and CSF.  Cells were isolated from the brain tumor at the time of autopsy and were cultured in neurobasal medium after mechanical disruption.
Clinical Data 20 months old male
Antigen Expression epithelial membrane antigen (EMA1), expressed
Comments

This cell line has been continuously carried in culture for over 8 months, and in neurobasal medium grows as spheres of varying sizes.

ATCC has verified that CHLA-04-ATRT expresses epithelial membrane antigen (EMA1) and does not express INI-1.

The generation of this cell line was made possible with the support of the Michael Hoefflin Foundation for Children's Cancer to Children’s Hospital Los Angeles, with the goal of making pediatric brain tumor lines available to the research community.

Complete Growth Medium DMEM:F12 Medium (ATCC 30-2006) with 20 ng/mL human recombinant EGF, 20 ng/mL human recombinant basic FGF, and B-27 Supplement (Invitrogen, Cat. No.17504) to a final concentration of 2% (v/v)
Subculturing

Cultures can be maintained by the addition of fresh medium when there are numerous, healthy-appearing clusters present in suspension and pH of medium has decreased. Alternatively, cultures can be established by centrifugation with subsequent resuspension in ¼ volume of the conditioned medium and ¾ volume fresh medium.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended.
Medium renewal: Add fresh medium twice weekly (depending on cell density).

Cryopreservation
Freeze medium: Fetal bovine serum, 90%; DMSO, 10% (v/v).
Cell culture tested DMSO is available as ATCC 4-X
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
D5S818: 12
D13S317: 12
D7S820: 11
D16S539: 11
vWA: 17
TH01: 6, 9
Amelogenin: X Y
TPOX: 8
CSF1PO: 11, 13
Name of Depositor A Erdreich-Epstein, Saban Research Institute, Children’s Hospital Los Angeles
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
峡江县| 姜堰市| 镇赉县| 柳州市| 五寨县| 进贤县| 九江县| 抚宁县| 乡宁县| 苏州市| 南昌县| 铜陵市| 濮阳市| 南昌县| 株洲县| 中牟县| 申扎县| 乐亭县| 磐石市| 新河县| 潢川县| 建宁县| 冕宁县| 宁城县| 卓尼县| 丹巴县| 边坝县| 咸丰县| 鹿邑县| 莫力| 宿州市| 沁阳市| 华宁县| 剑河县| 阳新县| 兖州市| 乐都县| 中山市| 富锦市| 达尔| 夏河县|