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A 1.195 kb BglII/HindII fragment contains the complete coding sequence and has been used as a probe.

Construct size (kb): 4.599999904632568

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: XhoI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IL8RB
Gene name: interleukin 8 receptor, beta
Contains complete coding sequence?: Y
Chromosome: 2; Localization: 2 q35
Tissue: HL-60 cell line
Disease: promyelocytic leukemia
Type of DNA: cDNA
Insert 5' end: Modification: EcoRI linker
Insert 3' end: Modification: XhoI linker
Insert size (kb): 1.7
Cross references: DNA Seq. Acc.: M73969
Nucleotides 165-1675 of the insert correspond to
nucleotides 1-1510 of M73969.

Insert lengths(kb): 1.700000047683716

Tissue: HL-60 cell line promyelocytic leukemia

Disease: promyelocytic leukemia

Gene product: interleukin 8 receptor, beta [IL8RB]

Restriction digests of the clone give the following sizes (kb): EcoRI--4.7; XhoI--4.7; EcoRI/XhoI--2.9, 1.8; PstI--4.7; BglII/HindIII--3.5, 1.2.

Includes 156 nt in addition to the 5' untranslated region reported in GenBank M73969. Nucleotides 1-9 of the insert are linker.

A 1.195 kb BglII/HindII fragment contains the complete coding sequence and has been used as a probe.