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produces protein interferon, beta 1, fibroblast

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: unknown
Intact vector size:
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
Cross references:

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: unknown
Intact vector size:
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: IFNB1
Other gene symbols: IFNB
Genomic copy number: unique
Gene name: interferon, beta 1, fibroblast
Contains complete coding sequence?: U
Chromosome: 9; Localization: 9 p22
Tissue: fibroblast
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 0.6
Cross references:

DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: IFNB1
Other gene symbols: IFNB
Genomic copy number: unique
Gene name: interferon, beta 1, fibroblast
Contains complete coding sequence?: U
Chromosome: 9; Localization: 9 p22
Tissue: fibroblast
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 0.6
Cross references:

Insert lengths(kb): 0.6000000238418579

Tissue: fibroblast

Gene product: interferon, beta 1, fibroblast [IFNB]

Target Gene: interferon, beta 1, fibroblast

Insert contains an internal PstI site.

Restriction digests of the clone give the following sizes (kb): BamHI/BglII--2.2, 0.6; PstI--1.8, 0.96; BamHI--2.8.

Encodes a 20 kDa protein product in Escherichia coli, corresponding to the expected molecular weight of unglycosylated interferon beta. Demonstrates anti-viral activity (inhibition of cytopathic effect using VSV and human fibroblasts).

Constructed by inserting a lacZ promoter (derivative of lac95 portable promoter) upstream of Bal31-digested pre-interferon beta cDNA.