產(chǎn)品名稱 |
pSP2-2 |
商品貨號 |
B233231 |
Designations |
pSP2-2 |
GenBank Number |
M25824
|
Species |
Homo sapiens, human |
Vector |
Construct size (kb): 3.900000095367432 |
Insert |
DNA: genomic Insert lengths(kb): 0.925000011920929 Tissue: leukocyte Gene product: ATPase, Ca++ transporting, plasma membrane 1 [PMCA1] |
Insert Size (kb) |
0.925 |
Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information |
Distributed: freeze-dried |
Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--3.9; BamHI/EcoRI--3.0, 1.0; BglI--3.9; BglI/PstI--2.5, 1.5; BglI/PvuII--2.8, 1.2. The insert contains the following restriction sites (nt from the 5' end): BalI--267; XmnI--696. Contains a constitutively spliced exon encoding amino acids 1057-1117, the following intron, and an alternatively spliced exon which contains 2 internal donor sites. |
References |
Olson S, et al. Localization of two genes encoding plasma membrane Ca2+ -transporting ATPases to human chromosomes 1q25-32 and 12q21-23. Genomics 9: 629-641, 1991. PubMed: 1674727
Strehler EE, et al. mRNAs for plasma membrane calcium pump isoforms differing in their regulatory domain are generated by alternative splicing that involves two internal donor sites in a single exon. Proc. Natl. Acad. Sci. USA 86: 6908-6912, 1989. PubMed: 2528729
|