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OL202 [OL 202]
OL202 [OL 202]
規(guī)格:
貨期:
編號(hào):B233368
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 OL202 [OL 202]
商品貨號(hào) B233368
Designations OL202 [OL 202]
Species Homo sapiens, human
Depositors C Wappenschmidt
Applications
Only insert DNA should be used for nick translation.
Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HindIII, MspI, PvuII, TaqI, XbaI. Tested against DNA from 9 unrelated Caucasians.
Vector
Construct size (kb): 5.800000190734863
Insert
DNA: cDNA
Insert lengths(kb): 0.6000000238418579
Tissue: lymphocyte
Gene product: DNA Segment, single copy [D12S9]
Alleles: A2, A1
Insert Size (kb) 0.600
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.2, 0.6; PstI--5.4, 0.45; BamHI--5.8; PvuII--2.9, 2.9; SalI--5.8.
Detects fragments of different chromosomal origin; polymorphic bands on chromosome 12, constant bands on chromosome 5, 12, 13, and 17.
Codominant segregation demonstrated in 4 informative families with 20 individuals.
Digest with BglII gives the following constant bands: 30, 18, 13, 9.6, 8.2, 3.5, 3, 2.6, 2.
Only insert DNA should be used for nick translation. Genomic fragments should be separated on 0.9% gels. Washing conditions: 0.1X SSC at 63-65C.
Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HindIII, MspI, PvuII, TaqI, XbaI. Tested against DNA from 9 unrelated Caucasians.
References

Wappenschmidt C, et al. Human cDNA probe detects RFLP on chromosome 12, OL 202 (HMG8 designated no. D12S9). Nucleic Acids Res. 14: 9221, 1986. PubMed: 2878418

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周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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