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pJY4313 [JY4313]
pJY4313 [JY4313]
規(guī)格:
貨期:
編號:B234487
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pJY4313 [JY4313]
商品貨號 B234487
Designations pJY4313 [JY4313]
GenBank Number

M74122

Species Streptococcus pneumoniae (Klein) Chester
Depositors Univ. Alabama, DE Briles, JL Yother, Univ. Alabama
Applications
produces protein pneumococcal surface protein A, 84 kDa
Vector
Construct size (kb): 4.599999904632568
Insert
DNA: genomic
Insert lengths(kb): 2.085999965667725
Gene product: pneumococcal surface protein A, 84 kDa [pspA]
Target Gene: pneumococcal surface protein A, 84 kDa
Insert Size (kb) 2.086
Media ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): HindIII/KpnI--2.7, 2.1; PvuII--2.4, 1.3, 0.96; SacI--3.5; 1.3.
Insert contains the following restriction sites (approximate kb from the 5' end): PvuII--1.13, 1.19; SacI--0.79.
The plasmid affects the growth of transformed bacteria and viability in stationary phase. Growth at 30C reduces product degradation.
The following primers can be used to amplify a 0.678 kb pspA product from the insert or genomic DNA (nt 1312-1990 of the published sequence): LSM1 5'-CCGGATCCAGCTCCTGCACCAAAAC-3'; LSM2 5'-GCGCTGCGACGGCTTAAACCCATTCACCATTGG-3'.
References

Briles DE, et al. Structural gene of pneumococcal protein. US Patent 5,476,929 dated Dec 19 1995

Yother J, et al. Truncated forms of PspA that are secreted from Streptococcus pneumoniae and their use in functional studies and cloning of the pspA gene. J. Bacteriol. 174: 610-618, 1992. PubMed: 1729250

David E Briles, personal communication

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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