国产精品乱视频,国产精品va久久久久久久,2021国产精品视频网站,91久久成人免费,乱色熟女少妇一区二区,天天干夜夜操夜夜操,麻豆久久樱花一区二区av,国产高清久久久久

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > EBO-pSV2-neo
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
EBO-pSV2-neo
EBO-pSV2-neo
規(guī)格:
貨期:
編號(hào):B239996
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 EBO-pSV2-neo
商品貨號(hào) B239996
Designations EBO-pSV2-neo
Depositors RF Margolskee
Biosafety Level 1
Vector Information
Size (kb): 13.3599996566772500
Vector: EBO-pSV2-neo (plasmid)
Promoters: Promoter SV40 late
Construction: pSV2neo, p220-1 (EBV oriP, EBNA-1, hph)
Marker(s):G418R,ampR,hygR
Construct size (kb): 13.35999965667725
Features: marker(s): ampR, hygR, G418R
promoter: SV40 late
replicon: pMB1, EBV oriP
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): NotI--uncut; BssHII--13.4; SfiI--7.5, 6.1; PstI--7.4, 2.3, 1.5, 1.0; SacI--13.4; BamHI--6.8, 5.4; HindIII--13.4; BglII--6.6.
pcD cDNA libraries can be converted into EBO-pcD libraries by ligating the 7.4 kb SfiI fragment of EBO-pSV2-neo (containing oriP, EBNA-1, hph) into SfiI-linearized, phosphatased, pcD DNA.
A selectable, stably replicating shuttle vector for expression of cDNAs in eukaryotic cells. Maintained episomally.
The vector contains EBV oriP and EBNA to ensure replication as episomes in human or simian cells, even if the cell line is not EBNA positive.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Margolskee RF, et al. Epstein-Barr virus shuttle vector for stable episomal replication of cDNA expression libraries in human cells. Mol. Cell. Biol. 8: 2837-2847, 1988. PubMed: 2841588

Mulligan RC, Berg P. Expression of a bacterial gene in mammalian cells. Science 209: 1422-1427, 1980. PubMed: 6251549

Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Genet. 1: 327-341, 1982. PubMed: 6286831

Okayama H, Berg P. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells. Mol. Cell. Biol. 3: 280-289, 1983. PubMed: 6300662

Mulligan RC, et al. Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome. Nature 277: 108-114, 1979. PubMed: 215915

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
临泽县| 互助| 武宣县| 迁安市| 台江县| 津市市| 漠河县| 大荔县| 石渠县| 曲松县| 来凤县| 轮台县| 鸡东县| 大宁县| 成武县| 安乡县| 上蔡县| 梁平县| 阿图什市| 阿拉善盟| 庆阳市| 望江县| 吴旗县| 娄底市| 新密市| 牟定县| 合山市| 县级市| 松江区| 宣武区| 西畴县| 大英县| 龙门县| 商都县| 霍山县| 蒲江县| 克山县| 马龙县| 台南市| 乌鲁木齐市| 西吉县|